Complete integrated workflow for viruses



Complete integrated workflow for viruses & parasites detection

Extraction platform & Amplification Thermal Cycler
  • All types of  food samples (ISO and non ISO)
  • ISO 15216-1&2 compliant
  • Process control
  • Nuclisens
  • Detection & quantification reagents

A complete integrated workflow = One Stop solution

For the detection of foodborne viruses Norovirus GI, Norovirus GII and Hepatitis A VIrus in Food samples, according to ISO15216 Standard, a unique range of service, equipment and reagents fit for purpose:

• Reliable and reproducible results, regardless of the technician thanks to calibrated products and positive internal control for each reaction.

• ISO 15216 recommended Process control Mengovirus

• Highly efficient extraction and acid nucleic acid purification with ISO 15216 recommended NucliSENS reagents

• Fast and Accurate Results with optimized GENE-UP PCR platform CEERAMTOOLS® real time RT-PCR kits compliant to ISO15216

• A large panel of RT-PCR and PCR kits for the detection of: Norovirus (Norovirus GI, Norovirus GII), Hepatitis Virus (Hepatitis A  virus, Hepatitis E virus),  Enterovirus, Astrovirus, Rotavirus, Adenovirus, Sapovirus as well as parasites (Cryptosporidium, Giarda)

• All-in-one, ready-to-use real time RT-PCR Kits validated on GENE-UP® and other real time PCR thermocyclers

• A range of unique services: training, support at local and international levels

• International recognized Expertise for foodborne virus testing

•Top-of-the-class Support by extremly skilles staff

ISO 15216 ScopeTOP

ISO 15216-1:2017 specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces.

Microbiology of food and animal feed -- Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR -- Part 1: Method for quantification

Microbiology of food and animal feed -- Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR -- Part 2: Method for qualitative detection

ceeramTOOLS Norovirus and Hepatitis A Virus RT-PCR real time kits comply to ISO/TS 15216-1 and 2

RT-PCR real time CEERAMTOOLS® Norovirus kits and CEERAMTOOLS® Hepatitis A Virus kit comply with ISO 15216-1:2017 and ISO/TS 15216-2:2013

KitsTOP

Ready-to-use

Ease-of-use (from tube to well)

All-in-one

IPC (Internal Positive Control) Included

- & + controls included

Mastermix & Enzyme mixes

Primers & Probes

Real Time PCR one step

9 to 18  months shelf-life

Bar coded for traceability

PackagingTOP

Norovirus kit and Hepatitis A Virus kit - as well as the all range of foodborne virus kits - are shipped with dry-ice in/with :

Optimized packaging for -15°C transportation

Compact sturdy space-saving packaging

Laboratory-Ready Protocols

WorkflowTOP

A complete integrated foodborne virus workflow for the detection and identification of both norovirus GI and Norovirus GII and hepatitis A Virus is key to better master the whole process and get top-of-the-class assistance :

  • A Complete integrated Workflow

1.      Process Control Mengovirus

2.      eGENE-UP Nucleic Acid Extraction Platform,

3.      NucliSens reagents of extraction and purification of Nucleic Acids

4.      PCR Real Time GENE-UP®Platform,

5.      Foodborne virus reagents (qualitative kits, quantitative kits)

  • Technical Assistance from highly qualified Foodborne virus Support Team
  • Adapted to other RT-PCR platforms (request assistance)

PerformancesTOP

Norovirus kit and hepatitis A Virus kit offer top performances for the detection and quantification of norovirus and hepatitis A Virus (as well as other viruses) :

95% amplification efficacy

Robustness (Reproducible and Repeatable)

Specificity 100%

Sensitivity LOD 5 genome copies per reaction*

100% compatible w/GENE-UP® real time PCR thermocycler

Standardized amplification

Fast run & Time saving

Optimized for routine analysis

From elution to result Intepretation

Quantification standards available

ISO 15216-1&2 Compliant

Only 5 µL input

ISO 15216 Scope

Soft Fruit Leaf, stem & bulb Vegetables Bottled Water    Food surfaces                  Bivalve Molluscan shellfish
Description Small edible Stoneless fruits Stem, Bulb, Leaf bi-valves filter feeders
Examples Strawberries, blackberries, blueberries, raspberries, goji berries or currants.. Spinach, green salads, onions, shallots, cilantro, etc Pepper, Surfaces Clams, Oysters, Mussels, cockles
Sampling 25 grams 25 grams up to 2 Liters 100 cm2 2 grams of digestive tissues

Non ISO 15216 Scope

Fruits eg Cherries, semi-dried tomatoes, pomegranate seeds, fruit preparations
Vegetables eg blanched leaf veggies, ready to eat food vegetables
Environmental samples eg sewage water, process water, irrigation water, drinking water, Sediments, Sludge, Surface Water, Groundwater, Subterranean Water, Recreational Water, Irrigation Water, Aquaculture
Herbs and spices eg pepper, powdered mint
 Processed foods eg cooked shellfish, paella
Beverages eg smoothies, cranberry juice
Meat  eg. mixed pork liver sausages, figatellu (raw pork liver dried) sausage, game
Dairy eg berry yogurt, berry ice cream, milk
Ordering information
  • External Process Control Mengovirus
  • Nucleic Acid Extraction and Purification NucliSens
  • ceeramTools Foodborne virus detection and identification
  • ceeramTools Virus Quantification
 
MICROORGANISMS item # DETECTION TESTS item # QUANTIFICATION (150µL)
1/ External Process Control 
Mengovirus KMG 50
2/ Nucleic Acid Extraction and Purification NucliSens
NucliSENS® Extraction Buffer 1 280130 4x11
NucliSENS® Extraction Buffer 2 280131 4x11
NucliSENS®  Extraction Buffer 3 280132 4x11
NucliSENS® Magnetic Sillica 280133
NucliSENS® Lysis Buffer(1000ml) 280134 4x11
3/ CeeramTools Foodborne virus detection and identification
Norovirus GI KNVGI 48 KQNVGI
Norovirus GII KNVGII 48 KQNVGII
Hepatitis A Virus KHAV 48 KQHAV
Hepatitis E Virus KHEV 48 KQHEV
Norovirus GI & GII KNVGIGII 48 KQNVGI & KQNVGII
Rotavirus KRV 48 KQRV
Adenovirus 40/41 KADV 48 KQADV
Adenovirus consensus KADVC 48 Not available
Astrovirus KASV 48 KQASV
Enterovirus KENV 48 KQENV
Sapovirus KSAV 48 Not available
Parasites detection
Giardia KGIAR 48 Not available
Cryptosporidium KCRYPT 48 Not available
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