Complete integrated workflow for viruses & parasites detection
Extraction platform & Amplification Thermal Cycler- All types of food samples (ISO and non ISO)
- ISO 15216-1&2 compliant
- Process control
- Nuclisens
- Detection & quantification reagents
A complete integrated workflow = One Stop solution
For the detection of foodborne viruses Norovirus GI, Norovirus GII and Hepatitis A VIrus in Food samples, according to ISO15216 Standard, a unique range of service, equipment and reagents fit for purpose:
• Reliable and reproducible results, regardless of the technician thanks to calibrated products and positive internal control for each reaction.
• ISO 15216 recommended Process control Mengovirus
• Highly efficient extraction and acid nucleic acid purification with ISO 15216 recommended NucliSENS reagents
• Fast and Accurate Results with optimized GENE-UP PCR platform CEERAMTOOLS® real time RT-PCR kits compliant to ISO15216
• A large panel of RT-PCR and PCR kits for the detection of: Norovirus (Norovirus GI, Norovirus GII), Hepatitis Virus (Hepatitis A virus, Hepatitis E virus), Enterovirus, Astrovirus, Rotavirus, Adenovirus, Sapovirus as well as parasites (Cryptosporidium, Giarda)
• All-in-one, ready-to-use real time RT-PCR Kits validated on GENE-UP® and other real time PCR thermocyclers
• A range of unique services: training, support at local and international levels
• International recognized Expertise for foodborne virus testing
•Top-of-the-class Support by extremly skilles staff
ISO 15216 ScopeTOP
ISO 15216-1:2017 specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces.
Microbiology of food and animal feed -- Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR -- Part 1: Method for quantification
Microbiology of food and animal feed -- Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR -- Part 2: Method for qualitative detection
ceeramTOOLS Norovirus and Hepatitis A Virus RT-PCR real time kits comply to ISO/TS 15216-1 and 2
KitsTOP
Ready-to-use
Ease-of-use (from tube to well)
All-in-one
IPC (Internal Positive Control) Included
- & + controls included
Mastermix & Enzyme mixes
Primers & Probes
Real Time PCR one step
9 to 18 months shelf-life
Bar coded for traceability
PackagingTOP
Norovirus kit and Hepatitis A Virus kit - as well as the all range of foodborne virus kits - are shipped with dry-ice in/with :
Optimized packaging for -15°C transportation
Compact sturdy space-saving packaging
Laboratory-Ready Protocols
WorkflowTOP
A complete integrated foodborne virus workflow for the detection and identification of both norovirus GI and Norovirus GII and hepatitis A Virus is key to better master the whole process and get top-of-the-class assistance :
- A Complete integrated Workflow
1. Process Control Mengovirus
2. eGENE-UP Nucleic Acid Extraction Platform,
3. NucliSens reagents of extraction and purification of Nucleic Acids
4. PCR Real Time GENE-UP®Platform,
5. Foodborne virus reagents (qualitative kits, quantitative kits)
- Technical Assistance from highly qualified Foodborne virus Support Team
- Adapted to other RT-PCR platforms (request assistance)
PerformancesTOP
Norovirus kit and hepatitis A Virus kit offer top performances for the detection and quantification of norovirus and hepatitis A Virus (as well as other viruses) :
95% amplification efficacy
Robustness (Reproducible and Repeatable)
Specificity 100%
Sensitivity LOD 5 genome copies per reaction*
100% compatible w/GENE-UP® real time PCR thermocycler
Standardized amplification
Fast run & Time saving
Optimized for routine analysis
From elution to result Intepretation
Quantification standards available
ISO 15216-1&2 Compliant
Only 5 µL input
ISO 15216 Scope |
|||||
Soft Fruit | Leaf, stem & bulb Vegetables | Bottled Water | Food surfaces | Bivalve Molluscan shellfish | |
Description | Small edible Stoneless fruits | Stem, Bulb, Leaf | bi-valves filter feeders | ||
Examples | Strawberries, blackberries, blueberries, raspberries, goji berries or currants.. | Spinach, green salads, onions, shallots, cilantro, etc | Pepper, Surfaces | Clams, Oysters, Mussels, cockles | |
Sampling | 25 grams | 25 grams | up to 2 Liters | 100 cm2 | 2 grams of digestive tissues |
Non ISO 15216 ScopeFruits eg Cherries, semi-dried tomatoes, pomegranate seeds, fruit preparations Vegetables eg blanched leaf veggies, ready to eat food vegetables Environmental samples eg sewage water, process water, irrigation water, drinking water, Sediments, Sludge, Surface Water, Groundwater, Subterranean Water, Recreational Water, Irrigation Water, Aquaculture Herbs and spices eg pepper, powdered mint Processed foods eg cooked shellfish, paella Beverages eg smoothies, cranberry juice Meat eg. mixed pork liver sausages, figatellu (raw pork liver dried) sausage, game Dairy eg berry yogurt, berry ice cream, milk |
- External Process Control Mengovirus
- Nucleic Acid Extraction and Purification NucliSens
- ceeramTools Foodborne virus detection and identification
- ceeramTools Virus Quantification
MICROORGANISMS | item # DETECTION | TESTS | item # QUANTIFICATION (150µL) |
1/ External Process Control | |||
Mengovirus | KMG | 50 | |
2/ Nucleic Acid Extraction and Purification NucliSens | |||
NucliSENS® Extraction Buffer 1 | 280130 | 4x11 | |
NucliSENS® Extraction Buffer 2 | 280131 | 4x11 | |
NucliSENS® Extraction Buffer 3 | 280132 | 4x11 | |
NucliSENS® Magnetic Sillica | 280133 | ||
NucliSENS® Lysis Buffer(1000ml) | 280134 | 4x11 | |
3/ CeeramTools Foodborne virus detection and identification | |||
Norovirus GI | KNVGI | 48 | KQNVGI |
Norovirus GII | KNVGII | 48 | KQNVGII |
Hepatitis A Virus | KHAV | 48 | KQHAV |
Hepatitis E Virus | KHEV | 48 | KQHEV |
Norovirus GI & GII | KNVGIGII | 48 | KQNVGI & KQNVGII |
Rotavirus | KRV | 48 | KQRV |
Adenovirus 40/41 | KADV | 48 | KQADV |
Adenovirus consensus | KADVC | 48 | Not available |
Astrovirus | KASV | 48 | KQASV |
Enterovirus | KENV | 48 | KQENV |
Sapovirus | KSAV | 48 | Not available |
Parasites detection | |||
Giardia | KGIAR | 48 | Not available |
Cryptosporidium | KCRYPT | 48 | Not available |